![]() ![]() They are general-purpose (basal) media, enriched media, selective or enrichment media, differential or indicator media, transport media, anaerobic media, and assay media. To meet these needs, numerous media are available.īased on their practical use, there are six types of standard culture media in microbiology laboratories. Many special-purpose media are needed to facilitate the recognition, enumeration, and isolation of certain types of bacteria. Bacteria having relatively basic and straightforward nutritional requirements are easy to grow in a lab, they are known as nonfastidious bacteria. Special nutritional supplements must be added to culture media to grow such bacteria. To enrich and/or transport clinical materialīacteria with complex nutritional requirements are called fastidious bacteria.To study metabolism, toxin, and enzyme production.To revive bacteria from lyophilized or stock culture.To grow bacteria for inoculum production for antibiogram testing.Broth medium serves various purposes such as propagation of many organisms, fermentation studies, and various other tests. Commonly used liquid media in the lab are nutrient broth, glucose broth, brain-heart infusion (BHI) broth, alkaline peptone water (APW), tryptic soy broth (TSB), and selenite F broth. These media contain specific amounts of nutrients but don’t have a trace of gelling agents such as gelatin or agar. ![]() Motility test medium, Stuart’s and Amies transport media, etc., are semisolid media. Semisolid medium has a soft custard-like consistency and is helpful for the cultivation of microaerophilic bacteriaor for determining bacterial motility. Semisolid culture media are prepared with agar at 0.5% or less concentrations.
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